Porcine BNP-32 EIA Kit 1 Kit (96 Wells) - 1 Kit

The immunoplate in this kit is pre-coated with secondary antibody and the nonspecific binding sites are blocked. The secondary antibody canbind to the Fc fragment of the primary antibody (peptide antibody)whose Fab fragment will be competitively bound by both biotinylatedpeptide and peptide standard or targeted peptide in samples. The biotinylatedpeptideinteractswithstreptavidin-horseradishperoxidase(SA-HRP)whichcatalyzes the substrate solution.  The intensity of theyellow is directly proportional to the amount of biotinylated peptideSA-HRPcomplex but inversely proportional to the amount of thepeptide in standard solutions or samples. This is due to the competitive binding of the biotinylated peptide with the standard peptide orsamples to the peptide antibody (primary antibody). A standard curveof known concentration can be established accordingly. The unknownconcentration in samples can be determined by extrapolation to thisstandard curve. Application: Method:ELISA Species: Storage: