Pantothenic Acid Microbiological Assay Kit - 96 Wells

Pantothenic acid is the reacitve thiol function of CoA and ACP Pantothenic acid (vitamin B5) is synthesized by most microorganisms and plants from pantoic acid. The vitamin is an itegral part of 4â-phosphopantheine, which is a component of coenzyme A (CoA). CoA plays a key role in the metabolism of numerous compounds, especially lipids and the ultimate catabolic disposition of carbohydrates and ketogenic amino acids. About 80% of the vitamin in animal tissues is in CoA form, and the rest exists mainly as phosphopanthetheine and phosphopantethenate. Another essential role of pantothenic acid is its participation in the 4â-phosphopantheine moiety of acyl carrier protein (ACP), where the phosphodiester-linked prosthetic group uses the sulfhydryl terminus to exchange with malonyl-CoA to form an ACP-S malonyl thioester, which can chain elongate during fatty acid biosynthesis. Pantothenic acid deficiency is exceedingly rare. Because of its rarity, most information about pantothenic acid deficiency has been obtained from experiments: Pantothenic acid deficiency has been induced in humans by use of a metabolic antagonist, w-methyl pantothenic acid along with a pantothenic acid-deficient diet. Subjects became irascible and developed postural hypotension and rapid heart rate on exertion, epigastric distress with anorexia and constipation, numbness and tingling of the hands and feet. Because pantothenic acid is involved with so many vital processes in the body, it is not surprising that a broad number of complications might result from deficiency. From recent research it is known that the pantothenic acid derivative, pantethine (two molecules of pantetheine joined by a disulfide bond), has a hypocholesterolemic effect. A metabolic antagonist of pantothenic acid, pantoyl ?-amino butyric acid (called pantoyl-GABA), is widely used in Japan as an antidementia drug for treating cognitive impairments in pathological states such as Alzheimerâs disease, presumably through increasing cholinergic activity in vivo. Serum / plasma (EDTA-, citrat-) samples are diluted and added into the microtiter plate wells coated with Lactobacillus plantarum (ATCC Nr. 8014) which metabolizes pantothenic acid. The presence of pantothenic acid both in standards [STD] and samples gives a pantothenic acid-dependent growth response. After incubation at 37°C for 24 h, the growth of Lactobacillus plantarum is measured turbidimetrically at 610 - 630 nm (alternative at 540 - 550 nm) in an ELISA-reader. A dose response curve of absorbance unit (optical density, OD at 610 nm) vs. concentration is generated using the values obtained from standard. Pantothenic acid present in the patient samples is determined directly from this curve. Application: Method:Microbiological Assay Species: Sample Type:Serum, Plasma SStorage: Stability: Sensitivity:2.5 µg/l Time:48 Hours