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Topoisomerase I Assay Kit - 250 assays

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This assay kit contains reagents for routine detection of type I topoisomerase activity. DNA markers (included) allow unequivocal detection of the enzyme. The assay is specific for topoisomerase I; (eukaryotic type II topoisomerases require MgCl2 and ATP). The standard kit is supplied with EDTA containing reaction buffer (minus Mg++) in order to prevent nucleolytic nicking of the supercoiled substrate (although we note that some eukaryotic type I activities are stimulated by Mg++). This assay will work even with crude extracts. A detailed manual of instruction is included that describes how to run the gels to maximally resolve topoisomers for analysis of DNA unwinding.

12,345.67 € 12345.67 EUR 12,345.67 €

12,345.67 €

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